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. 2011 Mar 16;4:65. doi: 10.1186/1756-0500-4-65

Table 1.

Characteristics of 24 polymorphic microsatellite loci in Zatorska geese population

Locus GenBank Accesion No. Source species Repeat motif of sequenced clone Primer sequence (5'-3') Ref. Ta N A Allele size range (bp) RS PIC HO HE PHWE Null allele freq. PE PI Chromosome, location (bp) (E-value)
Aal μ1 U63689 Anser albifrons TG F: CATGCGTGTTTAAGGGGTAT
R: TAAGACTTGCGTGAGGAATA
11 55 96 4 81-91 1.29 0.21 0.16 0.23 0.012 0.151 0.203 0.612 No match
APH12 AJ515893 Anas platyrhynchos domesticus (GAAA)4A2 (GAAA)2 F: TTAGTAGCATGTCAGGTTTATT
R: GCTTGTAGACTTCAGAGTTC
22 58 96 2 155-157 1.81 0.35 0.34 0.45 0.033 -0.131 0.265 0.405 Gga2, 130340088 (5.0E-98)
APH13 AJ515894 Anas platyrhynchos domesticus (GA)10 F: CAACGAGTGACAATGATAAAA
R: CAATGATCTCACTCCCAATAG
22 55 96 2 163-165 1.85 0.35 0.36 0.46 0.027 0.133 0.268 0.398 Gga7, 4082663 (9.0E-56)
APH16 AJ515897 Anas platyrhynchos domesticus (CA)7 F: CCTTCTGAACCTTCGTAG
R: AAATATAGACTTTTGTCCTGAA
22 58 96 2 144-148 1.94 0.37 0.38 0.48 0.219 0.086 0.277 0.382 Gga4, 36804087 (7.0E-21)
APH20 AJ515901 Anas platyrhynchos domesticus (CA)9 F: ACCAGCCTAGCAAGCACTGT
R: GAGGCTTTAGGAGAGATTGAAAAA
22 60 96 4 140-150 1.94 0.45 0.48 0.49 0.363 0.006 0.436 0.304 Gga8, 2546006 (3.0E-56)
Bca μ1 AF025889 Branta canadensis (TA)15 (CA)10 F: TGCTTTTTACCCCCAGTGTTCT
R: AGAATCTGCTATATTATTTCCAGCTC
18 61 96 5 115-125 3.81 0.69 0.59 0.74 0.000 0.123 0.677 0.114 Gga12, 4593760 (3.0E-08)
Bca μ5 AF025893 Branta canadensis (CA)9 F: AGTGTTTCTTTCATCTCCACAAGC
R: AGACCACAATCGGACCACATATTC
18 62 96 2 197-201 1.06 0.05 0.06 0.05 1.000 -0.006 0.051 0.895 Gga1, 74604866 (6.0E-40)
Bca μ6 AF025894 Branta canadensis (CA)10 F: TTTAACCCAGTAGCCTATCATGTCA
R: GTCTGAAGATAATGCTGCATGGTT
18 60 96 2 141-149 1.18 0.14 0.15 0.16 0.496 0.023 0.127 0.727 Gga2, 55974019 (3.0E-63)
Bca μ7 AF025895 Branta canadensis (CA)7N5 (CA)7 (TTTA)4 F: TAGTTTCTATTTGCACCCAATGGAG
R: CGGTCCTGTCCTTGTGCTGTAA
18 61 96 2 171-175 1.11 0.10 0.09 0.10 0.225 0.084 0.090 0.812 Gga2, 30816660 (1.0E-14)
Bca μ8 AF025896 Branta canadensis (CA)8 F: CCCAAGACTCACAAAACCAGAAAT
R:ATGAAAGAAGAGTTAAACGTGTGCAA
18 58 96 4 155-159 2.52 0.52 0.47 0.61 0.003 0.132 0.471 0.238 No match
Bca μ9 AF025897 Branta canadensis (CA)9 F: CCCAGTTCCTCTCATTCTCCTT
R: AAACAGGGAGGTGAAAGTGCTT
18 61 96 3 104-116 2.03 0.41 0.48 0.51 0.782 0.023 0.339 0.340 Gga7, 22851442 (4.0E-11)
Bca μ10 AF025898 Branta canadensis (CA)9 F: ATGTAGCCATGAAAATTAAAAAATG
R: CCAGTATTAGCCGAAAAGATGA
18 60 96 2 102-104 1.66 0.32 0.29 0.40 0.008 0.165 0.247 0.441 Gga2, 111179712 (3.0E-09)
CAUD-G007 AY493252 Anas platyrhynchos domesticus (CAG)5 (GCA)5 F: ACTTCTCTTGTAGGCATGTCA
R: CACCTGTTGCTCCTGCTGT
17 61 96 3 116-122 1.32 0.23 0.24 0.25 0.558 0.008 0.217 0.587 No match
CAUD-G012 AY493257 Anas platyrhynchos domesticus (AC)10 F: ATTGCCTTTCAGTGGAGTTTC
R: CGGCTCTAAACACATGAATG
17 57 96 3 203-211 2.11 0.44 0.53 0.53 0.656 0.006 0.372 0.313 GgaU, NW_ 001477064.1 (1.0E-11)
CAUD-G013 AY493258 Anas platyrhynchos domesticus (AC)9 F: ACAATAGATTCCAGATGCTGAA
R: ATGTCTGAGTCCTCGGAGC
17 61 96 2 92-98 1.42 0.25 0.32 0.30 0.726 -0.036 0.205 0.542 Gga13, 14041755 (0.009)
CKW5 AY720919 Anser cygnoides AC F: CAAAGCCCGTCATAGCA
R: AAGTTTCGGTCTGGATTGA
21 57 96 2 236-240 1.03 0.03 0.03 0.03 1.000 -0.002 0.031 0.938 Gga3, 50882942 (2.0E-32)
CKW14 AY720927 Anser cygnoides (CCT)5 F: AACTGATCCGGCAGAAAACTAA
R: ACTTAGCATGCAGCTTCACAAA
9 60 96 2 221-223 1.77 0.34 0.48 0.44 0.348 -0.054 0.260 0.414 Gga17, 4593759 (4.0E-33)
CKW18 AY720929 Anser cygnoides (CAAAA)7 F: AATGTGCTGTGTCACATTCTCC
R: CATCATCCAACGATTCAGACAT
9 57 96 2 246-250 1.76 0.34 0.41 0.43 0.638 0.022 0.259 0.416 Gga4, 52015193 (1.0E-22)
CKW21 - Anser cygnoides (TTA)10 F: CAAGGTAGTCATAAACCCAGAACA
R: ACAAAACTAATGGCAGGAAAC
21 62 96 6 346-375 2.87 0.59 0.45 0.66 0.000 0.193 0.571 0.182 Gga1, 2666911 (2.0E-64)
CKW43 AY790340 Anser cygnoides (CA)11 F: TCCAAGGCTTACTTCCCAAG
R: CAGAAGACAGGCCTGCAAAT
9 62 20(M) 2 129-131 1.84 0.35 0.68 0.46 0.047 -0.215 - - GgaZ, 9308527
76 (F) 2 129-131 1.94 0.37 0.00 0.49 0.000 0.999 - - (0.008)
CKW47 AY790335 Anser cygnoides (T)8(TG)7 F: AACTTCTGCACCTAAAAACTGTCA
R: TGCTGAGGTAACAGGAATTAAAA
9 62 96 2 213-215 1.13 0.11 0.12 0.11 1.000 -0.022 0.099 0.792 Gga4, 68269490 (4.0E-13)
TTUCG1 U66089 Branta canadensis CA F: CCCTGCTGGTATACCTGA
R: GTGTCTACACAACAGC
25,35 58 96 2 113-115 1.32 0.21 0.28 0.24 0.351 -0.072 0.179 0.605 Gga11, 13347684 (1.0E-11)
TTUCG2 U66090 Branta canadensis GT F: GAGAGCGTTACTCAGCAAA
R: TCACTCTGAGCTGCTACAACA
211, 25,35 55 96 2 112-128 1.92 0.36 0.52 0.48 0.518 -0.037 0.275 0.386 No match
TTUCG5 U66093 Branta canadensis TCTAT F: GGGTGTTTTCCAACTCAG
R: CACTTTCCTTACCTCATCTT
212, 25,35 61 96 7 176-216 2.35 0.55 0.55 0.58 0.242 0.012 0.569 0.209 Gga1, 269486597 (6.0E-04)

Ref., References; Ta, annealing temperature; n, number of individuals genotyped; A, number of alleles; RS, allelic richness; PIC, polymorphism information content; HO observed heterozygosity; HE expected heterozygosity; PHWE, Hardy-Weinberg equilibrium test P-value; PE, probability of exclusion; PI, probability of identity; M, males; F, females.

1under the name WD136

2under the name WD206