Figure 1.

A representative ¹H-NMR spectrum of plasma taken at t = 15 h from one of the acute liver failure animals. Relevant peaks identified and used for analysis are as follows: Lactate CH3 @1.33 ppm doublet Pyruvate CH3 @ 2.38 ppm singlet; Alanine CH @1.48 ppm doublet Threonine CH3 @ 1.34 ppm doublet; Glycine CH2 @ 3.57 ppm singlet Leucine CH3 @ 0.96 ppm triplet; Isoleucine CH3 @ 1.01 ppm doublet Valine CH3 @1.04 ppm doublet; Tyrosine H3/H5 @ 6.91 ppm PhenyalalnineH4 @ 7.38 ppm multiplet doublet.