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. Author manuscript; available in PMC: 2012 Apr 25.
Published in final edited form as: Virology. 2011 Feb 26;413(1):47–59. doi: 10.1016/j.virol.2010.12.052

Fig. 2. The D101.12 isolate is resistant to VCV and has a stable phenotype.

Fig. 2

(A) D101.12 and D1/85.16 were first tested for VCV sensitivity alongside their respective parental viruses, CC101.6 and CC1/85, in a PBMC-based HIV-1 replication assay. The cultures were infected with each isolate in the presence of the indicated VCV concentration, and p24 production was measured after 7 days. The data points represent the means of two independent experiments ± SEM. (B) The VCV-resistant isolate D101.12 was then cultured for 19 weeks in PBMC from two or three random blood donors, in the absence of VCV. Viruses from this reversion culture were tested for VCV sensitivity. The sensitivity profiles of representative viruses D101.12R7, D101.12R8, D101.12R10, and D101.12R19 (from weekly passages 7, 8, 10, and 19, respectively) after 7 days are shown in comparison to D101.12 and CC101.6. The values shown are the means of at least three independent experiments ± SEM.