Table 1.
Nomenclature and properties of viruses used in this study
| Virus | Type | Origin of env subunits in clones |
Engineered gp41 mutations (*) | gp41 sequence pattern | |
|---|---|---|---|---|---|
| gp120 | gp41 | ||||
| CC1/85 | Parental isolate | NA | NA | NA | NA |
| CC101.6 | Input isolate | NA | NA | NA | NA |
| D101.12 (R#) | VCV-resistant isolate (Reversion isolate#) | NA | NA | NA | Patterns I and II (Pattern II ) |
| S | Parental clone | CC1/85 cl.6 | CC1/85 cl.6 | NA | NA |
| S+(G514V) | Mutant parental clone | CC1/85 cl.6 | CC1/85 cl.6 | G514V | Pattern I |
| S+(G514V, V535M) | Mutant parental clone | CC1/85 cl.6 | CC1/85 cl.6 | G514V, V535M | Pattern I |
| S+(M518V, F519L) | Mutant parental clone | CC1/85 cl.6 | CC1/85 cl.6 | M518V, F519L | Pattern II |
| S+(M518V, F519L, V535M) | Mutant parental clone | CC1/85 cl.6 | CC1/85 cl.6 | M518V, F519L, V535M | Pattern II |
| S+(V535M) | Mutant parental clone | CC1/85 cl.6 | CC1/85 cl.6 | V535M | Patterns I and II |
| R14 | VCV-resistant clone | D101.12 cl.14 | D101.12 cl.14 | NA | Pattern I |
| R14/S | Chimera | D101.12 cl.14 | CC1/85 cl.6 | NA | NA |
| R14/S+(G514V) | Mutant chimera | D101.12 cl.14 | CC1/85 cl.6 | G514V | Pattern I |
| R14/S+(G514V, V535M) | Mutant chimera | D101.12 cl.14 | CC1/85 cl.6 | G514V, V535M | Pattern I |
| R14/S+(M518V, F519L) | Mutant chimera | D101.12 cl.14 | CC1/85 cl.6 | M518V, F519L | Pattern II |
| R14/S+(M518V, F519L, V535M) | Mutant chimera | D101.12 cl.14 | CC1/85 cl.6 | M518V, F519L, V535M | Pattern II |
| R14/S+(V535M) | Mutant chimera | D101.12 cl.14 | CC1/85 cl.6 | V535M | Patterns I and II |
#
Isolates designated as D101.12R followed by a number were derived from the PBMC culture in which D101.12 was cultured for a prolonged period in the absence of VCV; the number indicates the number of weekly passages (see Fig. 2B).
*
Amino acid numbering is based on HXB2 Env. The columns list the origins of gp120 and gp41 subunits in chimeric viruses based on the reference clones of CC1/85 (CC1/85 cl.6 = S) and D101.12 (D101.12 cl.14 = R14). Isolate CC101.6 and clone R14 both contain the critical H308P change in the gp120 V3 region.
NA, Not applicable.