Table 2.
Primer sequences for qRT-PCR
| Gene symbols | Gene names | GeneBank accession # | Primer sequences |
|---|---|---|---|
| NDST1 | N-deacetylase/N-sulfotransferase 1 | NM_008306 | F: 5’-TGCTCTGTGTGATCTGAGGCTAG-3’ |
| R: 5’-GATATCAAGCCCACAGCCAGAC-3’ | |||
| NDST2 | N-deacetylase/N-sulfotransferase 2 | NM_010811 | F: 5’-AAGTCTTAGAGCAGAACCCAC-3’ |
| R: 5’-ACTATGGGAAAGAGATGGATC-3’ | |||
| NDST3 | N-deacetylase/N-sulfotransferase 3 | NM_031186 | F: 5’-AGCTAACAACCAAAGTCACAG-3’ |
| R: 5’-CCTATCAGAGGCAAAAGGATG-3’ | |||
| NDST4 | N-deacetylase/N-sulfotransferase 4 | NM_022565 | F: 5’-ACCCTCTCTGGCAGCGAAATTG-3’ |
| R: 5’-GTTCCCTGTTCCAGATAGGTAG-3’ | |||
| GLCE | glucuronyl C5-epimerase | NM_033320 | F: 5’-TGGTTTGGTTCACTTCTCTGAG-3’ |
| R: 5’-AACTCCTAAAGGTCAAACCTGC-3’ | |||
| FGF1 | fibroblast growth factor 1 | NM_010197 | F: 5’-GAACCTCACCAAAAGCTTTCTC-3’ |
| R: 5’-GTTTCTAATTTGCTGGGCACTTG-3’ | |||
| FGF2 | fibroblast growth factor 2 | NM_008006 | F: 5’-GAGTGTTTCTTCTTTGAACGAC-3’ |
| R: 5’-GAGTTTATACTGCC AGTTCG-3’ | |||
| GAPDH | glyceraldehyde-3-phosphate dehydrogenase | NM_008084 | F: 5’-CACTGAAGGGCATCTTGGGCTACAC-3’ |
| R: 5’-TTGGAGGCCATGTAGGCCATGAGGT-3’ |
The sizes of the qRT-PCR products are 105–120 bp