Table 4.
Quantification of the increases in specific HS glycoforms in MPS IIIB mouse brain cells
| Mice | IF intensity (grey value) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| HS4E4 | HS4C3 | EV3C3 | AO4B08 | RB4Ea | ||||||
| BS | CB | BS | TH | BS | CB | BS | CB | HP | CB | |
| +/+ | 130 ± 8 | 101 ± 7 | 105 ± 16 | 105 ± 7 | 134 ± 20 | 131 ± 10 | n/a | 122 ± 19 | 122 ± 14 | 149 ± 25 |
| −/− | 168 ± 19 | 248 ± 5 | 238 ± 10 | 247 ± 4 | 236 ± 26 | 248 ± 3 | 248 ± 4 | 249 ± 3 | 245 ± 2 | 246 ± 5 |
Quantitative analysis of IF images in Fig. 1. All non-vasculature IF signals were analyzed by manual thresholding using Image J software. Data here are the means±SD/unit (cell)
+/+ wt, −/− MPS IIIB, BS brain stem, CB cerebellum, TH thalamus, HP hippocampus, n/a no non-vasculature IF signal. IF intensity was significant between all compared groups (P < 0.01)