Table 5.

Elevated expression of genes essential for HS biosynthesis in MPS IIIB mouse brains

Genes	a2-∆∆Ct (fold of increase)
	b1 mo	b6 mo
NDST1	1.1	c1.7
NDST2	c1.4	c1.8
NDST3	c1.7	c2.1
NDST4	c2.0	c1.6
GLCE	c1.4	c1.7
FGF1	0.9	1.0
FGF2	c1.5	c1.3

^aqRT-PCR data was analyzes by means of the comparative threshold (Ct) method and expressed as 2^-∆∆Ct (fold of increase), relative quantitation of gene expression in the brain of MPS IIIB mice (n = 5) vs. wt mice (n = 4)

^bThe age of mice tested

^cThe increases in gene expressions are significant (P < 0.05, ∆Ct-MPS IIIB vs. ∆Ct-wt)