Figure 6.

Quantitative reverse transcription (RT)-PCR analysis of undifferentiated human embryonic stem cells (hESCs), early mesoderm progenitors (hEMP), and CD34^bright and CD34^dim cells. (a) Schematic model of stages of mesoderm and hematopoietic differentiation from hESCs under mesoderm-specific conditions [(bone morphogenetic protein-4 (BMP-4), basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) (no serum) (B+F+V)], showing immunophenotypic populations isolated for gene expression analysis by qRT-PCR. Day of isolation of each population is shown. Numbers 1–4 next to the boxes correspond to the populations analyzed by qRT-PCR in b–e. (b) Mesoderm-associated genes. (c) Genes associated with definitive hematopoietic development. (d) Positive regulators involved in B cell development and maturation. (e) Negative regulators that potentially block B cell development. Fold change in expression is shown on y-axis after normalization to housekeeping gene RPL-7. Mean ± SEM for three independent experiments using H9 cells.