Skip to main content
. 2011 Jan 11;19(4):642–649. doi: 10.1038/mt.2010.293

Figure 2.

Figure 2

Rhodopsin mRNA expression levels. (a) Contralateral eyes of adult P347S mice (n = 6) expressing a mutant human RHO transgene were subretinally injected with 6.0 × 108 vp AAV-S or AAV-R. Two weeks postinjection retinas were harvested, transduced (green) cells collected by fluorescence-activated cell sorting (FACS) and RNA extracted. The level of human RHO suppression by AAV-S, determined by qPCR using human-specific RHO primers, was 68 ± 2.4%. (b) Adult wild-type mice (n = 7) were subretinally injected with a mixture of either 6.0 × 108 vp AAV-S and 1.8 × 1010 vp AAV-R or 6.0 × 108 vp AAV-C and 1.8 × 1010 vp AAV-R. Two weeks postinjection total RNA was extracted and levels of replacement RHO RNA determined by qPCR. No significant difference in replacement transcript levels was observed in eyes that received AAV-R and AAV-S versus AAV-R and AAV-C, indicating that no significant suppression of the replacement transcript had occurred (P = 0.814). (c) Adult wild-type mice (n = 8) were subretinally injected with AAV-R. Total RNA was extracted 2 weeks postinjection and levels of RHO replacement expression from AAV-R compared to levels of RHO in normal human rhodopsin (NHR) mouse retinas. Levels of RHO mRNA expression from the AAV-R was 31 ± 5% of levels in NHR mouse retinas.