Figure 2.

Reovirus induces homing of immune cell into prostate tumors. C57BL/6 mice-bearing transgenic adenocarcinoma of mouse prostate-C1 (TRAMP-C1) tumors were administered with therapeutic regimen of phosphate-buffered saline (PBS)/UV-inactivated reovirus (UVRV)/live reovirus (LRV). On day 3 post last injection (p.l.i.), tumor-infiltrating lymphoid cells (TILs) were isolated, stained, and directly analyzed in flow cytometry to determine the frequency of (a) H2Kb⁺ immune cells, (b) CD3⁺, CD8⁺ T cells, and (c) NK cells. Numbers listed on dot-plots indicate the percentages of cells present in a respective quadrant, while bar graphs represent the cumulative data from three independent experiments for respective cell subtype.