Figure 3.

Attraction of lymphocytes populations to vvCCL5-infected tumors. (a) Mice (C57/BL6 bearing MC38 tumors) were treated via intraperitoneal injection of phosphate buffered saline (PBS), vvDD or vvCCL5. Tumors were dissociated and single cells stained with anti-CD4 antibody to determine the percentage of CD4⁺ cells in the tumor or anti-CD11c to look at dendritic cell (DC) infiltration (left panel) or tumor sections stained with antibodies to CD11c⁺ (DC; red) or CD4⁺ (green). (b) Immunohistochemistry of MC38 tumor sections following treatment with PBS of vvCCL5 reveals the levels of attraction of CD4⁺ T-cells (white) and CD4⁺FoxP3⁺ cells (black bars) as determined from 10 independent regions from three sections from three individual tumors. Representative sections at day 3 are shown (CD4⁺ (red) and FoxP3 (green)). (c) Relative attraction of cytokine-induced killer (CIK) (natural killer T) cells to lymphomas infected with different viruses. Mice (friend virus B (FVB)) bearing 6814 bilateral tumors) were treated through intravenous injection with CIK cells expressing luciferase. After 24 hours, the bilateral tumors were injected directly with different viruses, and 48 hours later bioluminescence imaging was used to determine the relative infiltration of each tumor with labeled CIK cells. Left panel; relative attraction of CIK cells to mock infected versus vvCCL5-infected tumors. Right panel; relative attraction of CIK cells to vvDD versus vvCCL5-infected tumors. (P < 0.05). BLI, bioluminescence imaging.