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. 2011 Feb 1;19(4):782–789. doi: 10.1038/mt.2010.314

Figure 3.

Figure 3

Efficient reprogramming by the novel vector system. (a) Schematic illustration of reprogramming by virus-encoded transduction of Oct4-EGFP (OG2) transgenic murine embryonic fibroblasts (MEFs). (b) Representative flow cytometry analysis of Oct4-EGFP expression in OG2 MEFs 11 days post-transduction with all 4 factors (lower) and their untransduced counterparts (upper). (c) Fluorescence microscopy of selected OG2-derived induced pluripotent stem cell (iPSC) clones demonstrating embryonic stem cell morphology and Oct4-EGFP activation. Upper brightfield, middle EGFP fluorescence, lower overlay.