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. 2011 Feb 1;19(4):782–789. doi: 10.1038/mt.2010.314

Figure 5.

Figure 5

Exemplary sequence of induced pluripotent stem cell (iPSC)-like colony development and associated marker expression. A single fibroblast (1) (a) transduced with a 4-in-1 reprogramming vector (linked to nuclear-localized dTomato), (b) gives rise to progeny (serially numbered and tracked in orange, also see tracking tree in Supplementary Figure S8), (c) that adopts an iPSC-like morphology. (d) Consecutively, an Oct4-EGFP reporter switches on in a subset of cells. Left phase contrast; middle nuclear-localized dTomato; right Oct4-EGFP signal. (e) An overlay is shown of the dTomato-nucMembrane signal and the Oct4-EGFP of the same colony as in d.