Figure 2.

Association between lentiviral integration and gene expression in mouse fibroblasts, mouse eye, and brain tissues. Illumina BeadChip microarray analyses of untransduced dividing and postmitotic mouse cell types revealed a slightly preferred vector integration into (highly) expressed cellular genes for dividing mouse SC-1 fibroblasts (dark blue). The integration preference into expressed genes is less pronounced for mouse postmitotic eyes and brain cells (light blue). The respective Probe IDs with “NM_” accessions on the Illumina MouseRef-8 and Illumina MouseRef-6 Expression BeadChip were grouped and sorted on their expression values (four BINs), (0: likely background expression; 1: low expression; 2: medium expression; 3: medium to high expression). The ratio of integration site within RefSeq genes belonging to one of these four groups to the percentages of overall RefSeq genes which are belonging to these four groups are displayed.