Figure 1.

Wnt11 expression in sorted mouse muscle-derived stem cells (MDSCs). (a) Schematic illustration of feline leukemia virus (FLV)-based lentiviral vectors used in our experiments. The vectors contain human cytomegalovirus (CMV) immediate-early enhancer/promoter, enhanced green fluorescent protein (eGFP) as an expression reporter and neomycin (Neo) as a selection marker, and Wnt11 gene was inserted into the downstream of eGEP gene. (b) Immunofluorescent (IF) staining of Wnt11 revealed a significant increase in the expression of Wnt11 in MDSCs transduced with Lv-Wnt11 at 2 weeks after infection, whereas low levels of Wn11 expression was also detected in the control cells (magnification: ×200). (c) Semiquantitative RT-PCR analysis showed that Lv-Wnt11 transduction increased the expression of Wnt11 mRNA in MDSCs 2 weeks after infection. Fifty nanogram of RNA extracted from MDSCs by Trizol reagent was used as the template for reverse transcription (RT)-PCR.