Table 1.
List of Antibodies Used for Immunohistochemical Staining
| Antibody | Host | Dilution | Manufacturer | Specificity |
|---|---|---|---|---|
| Sagittal sections | ||||
| Human nuclei | Mouse | 1:200 | Chemicon | Human nuclei |
| GFAP | Rabbit | 1:2000 | Chemicon | Astrocytes |
| NG2 | Rabbit | 1:250 | Chemicon | CSPG (NG2) deposition |
| SMI-31R | Rabbit | 1:1000 | Covance | General fiber growth |
| 5-HT | Rabbit | 1:20,000 | Immunostar | Serotonergic fibers |
| CGRP | Rabbit | 1:2000 | Peninsula | Sensory fibers |
| Ki67 | Rabbit | 1:1000 | Chemicon | Cell proliferation |
| Olig2 | Rabbit | 1:500 | Chemicon | Oligodendrocytes |
| Cross sections | ||||
| CGRP | Rabbit | 1:2000 | Peninsula | Sensory fibers |
| 5-HT | Rabbit | 1:20,000 | Immunostar | Serotonergic fibers |
| GAP-43 | Mouse | 1:50,000 | Chemicon | Axonal growth |
| VR-1 | Guinea pig | 1:5000 | Chemicon | Unmyelinated C-fibers |
GFAP, glial fibrillary acidic protein; 5-HT, serotonin; CGRP, calcitonin gene-related peptide; VR-1, vanilloid receptor type 1; CSPG, chondroitin sulfate proteoglycan.
Chemicon International, Temecula, CA; Immunostar, Hudson, WI; Peninsula Laboratories, San Carlos, CA; Covance Inc., Princeton, NJ.