FIG. 4.

(A) Comparison of the TUNEL-positive cells among the striatal subregions. There were no significant differences in the numbers of TUNEL-positive cells among the five subregions in the striatum (n=10; DL, dorsolateral; VL, ventrolateral; VM, ventromedial; DM, dorsomedial; C, central). (B) Double staining showed that most of the TUNEL-positive cells (green) co-localized with NeuN (neuronal marker, red); however, they did not co-localize with GFAP (astrocyte marker), or Iba-1 (microglia marker, red). Nuclei were counterstained with DAPI (blue; magnification×400; scale bar=20 μm; TUNEL, terminal deoxynucleotidyl transferase-mediated uridine 5′-triphosphate-biotin nick-end labeling; NeuN, anti-neuron-specific nuclear protein; GFAP, anti-glial fibrillary acidic protein; DAPI, 4′,6-diamidino-2-phenylindole; Iba-1, anti-ionized calcium-binding adaptor molecule 1). Color image is available online at www.liebertpub.com/neu.