(A and B) Quantitative evaluation of oxidative protein damage by detection of the carbonyl groups introduced into proteins. The levels of the carbonyl groups in the striatum significantly increased 24 h after bilateral common carotid artery occlusion (BCCAO) compared with sham animals (n=6; *p<0.05). (C and D) Representative photomicrographs of 8-oxoG staining. Expression of 8-oxoG increased in the striatum 24 h after BCCAO. 8-oxoG staining co-localized with DARPP-32 staining; however, it did not co-localize with ChAT staining (magnification×400; scale bar=50 μm;. I and Isc, ischemia; 8-oxoG, anti-8-oxoguanine; DARPP-32, anti-dopamine and cyclic AMP-regulated phosphoprotein of relative molecular weight 32,000; ChAT, anti-choline acetyltransferase). Color image is available online at www.liebertpub.com/neu.