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Effect of overexpression of the tri-alanine substitutions of σ70 R4 on growth at 42°C in strain PB1rpoD800.
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Several mutants conferred temperature-lethal phenotypes (T552A-R554A, D570-T572-Q579-D581) while others conferred partial defects (G564A-T566A, V576A-K578A, D588A-I590A). The mutants conferring growth defects are highlighted in grey. N.D., no dilution.
