Fig. 2.
Effects of SP-A on prostaglandin production by term DSCs. Term DSCs were isolated, purified, treated with 17β-estradiol (E2; 10−8 m), medroxyprogesterone acetate (MPA; 10−7 m), both, or vehicle for 7 d, and then stimulated with SP-A (1–100 μg/ml) for 24 or 48 h as described in Materials and Methods. Positive controls included IL-1β (1 ng/ml). Levels of PGF2α (A) and PGE2 (B) were measured in conditioned supernatant by specific ELISA and corrected for protein content. Results are shown for E2 + MPA pretreatment and 24-h incubation only. Data are presented as mean ± sem from a minimum of four separate experiments performed in triplicate. Statistical differences are shown.