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. Author manuscript; available in PMC: 2011 Sep 30.
Published in final edited form as: Nature. 2011 Mar 31;471(7340):647–650. doi: 10.1038/nature09918

Fig. 2.

Fig. 2

D-ornithine-dependent UAG translation requires cells with pylTSCD, but not pylB. E. coli bearing the indicated plasmids was induced for expression of mtmB1 and pyl genes then analyzed by immunoblot for full-length 50 kDa mtmB1 product (upper arrow), or 23-kDa amber-terminated mtmB1 product (lower arrow). E. coli transformed with pDLBADHis and: pK parent vector pACYC-Duet1 (lane 1), pK13 bearing pylB, pylC, pylD (lane2), pK14 bearing pylB, pylD (lane 3), pK15 bearing pylC, pylD (lane 4), or pK16 pylB, pylC (lane 5) were grown in the presence of D-ornithine.