Table 1.

PCR and DHPLC conditions for the mutational analysis of E-cadherin

	Annealing	Amplicon	DHPLC
	temperature	length	temperature
Exon no	(°C)	(bp)	(°C)
1	70	285	66
2	55	377	62
3	60	352	58, 61
4	60	351	60
5	60	398	58
6	58	246	59
7	60	329	60, 62, 64
8	58	225	59, 62
9	55	252	59
10	55	309	58
11	60	243	61
12	60	326	59, 61
13	58	297	58
14	60	206	61
15	60	244	58
16	60	318	59