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. Author manuscript; available in PMC: 2011 Apr 4.
Published in final edited form as: Proteomics Clin Appl. 2008 Jul 24;2(9):1234–1245. doi: 10.1002/prca.200780103

Figure 2.

Figure 2

Detection of antibodies elicited by UV-irradiated E. coli vector-based vaccine via antigen microarray and Western blot. Mouse sera were collected 6 and 9 weeks after intranasal immunization with an E. coli vector-based vaccine [E. coli BL21 (DE3) T7/lacO Tet-c] (Tet-c vector) or an empty vector without Tet-c insertion (Empty vector). Antigen microarrays were in-house fabricated as described in Section 2. Briefly, two duplicated purified Tet-c (0.7 μg) and IgG (0.7 μg; a positive control) were spotted on an individual poly-L-lysine slide. Antigen arrays were hybridized with mouse sera (A) and commercial Tet-c antibody (B) (100-fold dilutions). The production of Tet-c antibody was also confirmed by Western blot (C). Purified Tet-c (5 μg) was loaded into 10% SDS-PAGE. Tet-c transferred nitro-cellulose membranes were reacted with mouse sera, which were collected 9 weeks post-immunization with a Tet-c vector (lane 1) and an empty vector (lane 2). Data are representative of three separate experiments with similar results.