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. Author manuscript; available in PMC: 2012 Apr 25.
Published in final edited form as: Virology. 2011 Feb 25;413(1):1–11. doi: 10.1016/j.virol.2010.12.036

Figure 4. Cytokine-rich supernatants from activated T cells cannot enhance KSHV infection of tonsillar T cells or B cells.

Figure 4

Culture supernatants from mock-, PHA- or MLR-stimulated cells were collected at the indicated time points (1~4 days post-stimulation) and used as conditioned medium after filtered through 0.2-μm nylon filter. Cells from fresh tonsils were treated with medium, PHA (10 μg/ml) or conditioned medium, added at 40% v/v, for 24 hours and washed extensively before virus infection. At 48 hours post-infection, cells were stained and virus infectivity was analyzed on flow by assessing GFP expression in CD4+ (A), CD8+ (B), or CD19+ (C) cells. One representative data of 3 independent experiments is shown.