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. Author manuscript; available in PMC: 2012 Mar 17.
Published in final edited form as: Cell Host Microbe. 2011 Mar 17;9(3):235–242. doi: 10.1016/j.chom.2011.02.004

Figure 2. CHMP2 and CHMP4 Protein Families Are Required for HIV-1 Release and Infectivity.

Figure 2

(A,B) Viral titers, virion-associated Gag proteins, and intracellular protein levels in 293T cells expressing HIV-1 and depleted or co-depleted of the designated (A) CHMP2 or (B) CHMP4 family members. Figure panels are equivalent to those shown in Figure 1 (n = 3 titer measurements ± S.D.). (C,D) Thin-sectioned, transmission electron micrographs of arrested HIV-1 budding particles in cells lacking CHMP2A/B (C) or CHMP4A/B (D). Scale bars are 100 nm. (E) Quantification of arrested budding particles without (grey) or with (black) visible striations within their necks (see arrows in Figure 2C for examples). (see also Figure S2).