Skip to main content
. Author manuscript; available in PMC: 2011 Apr 4.
Published in final edited form as: J Immunol. 2010 Jan 8;184(4):1858–1866. doi: 10.4049/jimmunol.0903210

FIGURE 4.

FIGURE 4

IL-12 suppresses VEGFR3 on tumor vessels via IFN-γ and upregulates IFN-γ production by tumor infiltrating immune cells. A, B16 (solid lines) or B16/IL-12 (dotted lines) cells (2 × 105) were injected i.m. into IFN-γ−/− mice and thigh diameter measured over time. Each line represents one mouse. B, The proportion of vessel area that stained positive for VEGFR3 in the mice described in A was calculated using ImagePro software. The dotted line at 0.36 represents the level of staining seen in B16/IL-12 tumors grown in a WT mouse, which does not have VEGFR3 on its vessels and therefore represents nonspecific staining. Growth curves and VEGFR3 quantification represents pooled data from two experiments with a total of six animals per group. C, WT mice injected with 2 × 105 B16 or B16/IL-12 cells. Tumors were grown to a thigh diameter of 10 mm, weight-matched, and analyzed for IFN-γ production by intracellular cytokine staining. Each population was pregated on CD45+ cells. Significance was calculated using Mann-Whitney U test.