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. Author manuscript; available in PMC: 2012 Feb 25.
Published in final edited form as: Immunity. 2011 Feb 25;34(2):163–174. doi: 10.1016/j.immuni.2011.02.003

Figure 2.

Figure 2

Analysis of lymphoid lineages in RAG-2(T490A) mice. (A) Flow cytometric analysis of thymocytes from homozygous RAG-2 wild-type (WT/WT), neo-excised RAG-2 wild-type (WT*/WT*) and RAG-2(T490A) (T490A/T490A) mice. Upper panel, thymocytes assayed for CD4 and CD8; lower panel, thymocytes, gated on the CD4−CD8− population, assayed for CD44 and CD25. (B) Flow cytometric analysis of bone marrow cells, assayed for B220 and CD43, from homozygous RAG-2 wild-type (wt/wt), neo-excised RAG-2 wild-type (wt*/wt*) and RAG-2 T490A (T490A/T490A) mice. Percentages of cells within each quadrant are indicated. (C) Reduction of thymic cellularity in homozygous RAG-2 T490A mice, relative to wild-type. Circles (wt/wt) and squares (T490A/T490A) represent cellularity of thymuses from individual mice; horizontal bars indicate mean cellularity. Significance was estimated by the two-tailed t test. (D) Increased apoptosis among CD4−CD8− thymocytes from RAG-2 T490A mice. Symbols represent individual mice; horizontal bars indicate mean percentages. Genotypes are indicated in inset at right. Significant differences are indicated with confidence limits as determined by the two-tailed t test.