Figure 2.—

Sir3-dependent telomere silencing requires the protein degradation pathway. (A) The indicated strains were constructed in a reporter strain in which the URA3 gene is inserted approximately 2.1 kb away from the left end of the chromosome VII (Rundlett et al. 1996). Serial dilutions were plated on SC and SC + 5-FOA plates. (B) The indicated strains were constructed in a reporter strain in which the URA3 gene is placed at the very end of the chromosome V (kahana and gottschling 1999). Serial dilutions were plated on YPD, SC–Ura, and SC + 5-FOA plates.