Figure 7.—

Examples of loci identified by the pipeline and confirmed by 5′ and 3′ RACE. Part of the S. pombe genome represented by the X:Map genome browser (http://xmap.picr.man.ac.uk) is shown. The chromosome runs across the middle of the figure with genes on the forward strand shown above the center line and those on the reverse strand, below. Boxes represent genes, with the transcript structure and exons indicated by the smaller boxes within. (A) The 5′ extension to SPBC16H512c identified by two novel peptides. The original and revised gene structures are shown; the revised gene structure incorporates a locus previously annotated as a ncRNA (noncoding RNA). (B) Location of new10, enhancer of rudimentary homolog-like protein, identified by comparative genomics.