TABLE 1.
Novel genes detected by the pipeline
| Method | GeneDB accession | Assigned gene name | Description/product | Transcriptional evidence (PCR/RNASeq) | Deletion phenotype |
|---|---|---|---|---|---|
| CG | SPAC222.19 | tam1 | Sequence orphan | Yes/Yes | NA |
| CG | SPAC17G8.15 | new1 | Histone-like transcription factor | Yes/Yes | Slow growth |
| CG | SPAC1805.18 | new2 | Sequence orphan | Yes/Yes | NA |
| CG | SPAC1486.11 | new3, fmc1 | Mitochondrial matrix protein | Yes/Yes | NA |
| CG and PG | SPAC3H5.13 | new4 | Conserved eukaryotic protein | Yes/Yes | NA |
| CG and PG | SPAC15A10.17 | new5, coa2 | Cytochrome C oxidase assembly factor | Yes/Yes | Elongated cellular morphology |
| CG and PG | SPAC4F10.22 | tam2, cmc4 | Mitochondrial intermembrane space protein | Yes/Yes | Viable |
| CG | SPAC1B3.21 | tam3 | Mitochondrial conserved protein | Yes/Yes | NA |
| CG | SPRRNA.56 | NA | rRNA | Yes/Yes | NA |
| CG | SPAC9G1.15c | tam4, mzt1 | Mitotic-spindle organizing protein | Yes/Yes | NA |
| CG | SPAC13F5.08 | new6, vts1 | Vps20-associated protein | Yes/Yes | NA |
| CG | SPAC6B12.19 | new7, rsa3 | Ribosome assembly protein Rsa3 | Yes/Yes | NA |
| CG | SPAC3G9.17 | new8 | Holo-[acyl-carrier-protein] synthase | Yes/Yes | Slow growth |
| PG | SPAC16E8.18c | tam5 | Sequence orphan | Yes/Yes | NA |
| CG | SPAC926.10 | new9 | Sequence orphan | Yes/Yes | NA |
| CG | SPAC19G12.17 | new10 | Enhancer of rudimentary homolog | Yes/Yes | NA |
| PG | SPAC23A1.20 | new11 | Pseudogene, truncated protein, no N-term methionine | Yes/Yes | NA |
| CG | SPAC1F7.14 | tam6 | Mitochondrial conserved protein | Yes/Yes | NA |
| CG | SPAC4D7.15 | new12 | Sequence orphan | Yes/Yes | NA |
| CG and PG | SPAC4D7.14 | new13 | Conserved fungal protein | Yes/Yes | NA |
| PG | SPBC839.20 | new14 | Dubious | No/No | NA |
| CG | SPBC409.23 | tam7 | Conserved fungal protein | Yes/Yes | Slow growth |
| CG & PF | SPBC8D2.23 | new15 | Mitochondrial protein, ribosomal subunit L35 related | Yes/Yes | NA |
| CG | SPBC3H7.18 | tam8 | Sequence orphan | Yes/Yes | NA |
| CG | SPBC1711.18 | tam9 | Mitochondrial ribosomal protein subunit L36, MrpL36/YmL36 | Yes/Yes | NA |
| CG | SPBC24C6.13 | new16 | Conserved eukaryotic protein | Yes/Yes | NA |
| CG | SPBC32F12.16 | new17 | Sequence orphan | Yes/Yes | NA |
| CG | SPBC30D10.21 | new18 | Mitochondrial protein | Yes/Yes | NA |
| CG | SPBC887.22 | new19 | Signal peptidase complex subunit Spc1 | No/Yes | NA |
| CG | SPBC839.19 | new20 | Conserved eukaryotic protein, broadly conserved (to Metazoa) | Yes/Yes | NA |
| CG and PF | SPBC530.16 | new21, ksh1 | DUF1242 family protein | Yes/Yes | Inviable |
| CG and PG | SPBC14C8.19 | tam10 | Sequence orphan | Yes/Yes | NA |
| PG | SPBC17D1.17 | tam11 | Sequence orphan | Yes/Yes | Elongated cellular morphology |
| CG | SPBC1105.19 | tam12 | Sequence orphan | Yes/Yes | NA |
| CG | SPBC56F2.15 | tam13 | Sequence orphan | Yes/Yes | NA |
| PF | SPCP20C8.04 | new22 | DUF1773 (S. pombe specific family) | No/No | NA |
| CG | SPCC330.20 | tam14 | Sequence orphan—conserved outside Schizosaccharomyces | Yes/Yes | NA |
| CG | SPCC63.09 | new23 | Dubious | Yes/No | NA |
| CG, PG, PF | SPCC4B3.20 | new24, cmc2 | Copper-binding protein of the mitochondrial inner membrane Cmc1 | Yes/Yes | NA |
| CG | SPCC330.21 | new25 | Dubious | Yes/Yes | NA |
CG, comparative genomics; PG, proteogenomics; PF, Pfam scan.