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. Author manuscript; available in PMC: 2012 Mar 25.
Published in final edited form as: Immunity. 2011 Mar 3;34(3):315–326. doi: 10.1016/j.immuni.2011.01.013

Figure 1.

Figure 1

TCR CDR3β loop modulates αGC-CD1d tetramer binding. (a) Staining of hybridomas expressing the Vα14 TCRα chain paired with the Vβ8.2 chain in the context of the indicated CDR3β-Jβ rearrangement. The MFI of αGC-CD1d tetramer staining was determined for a narrow TCR gate. Data represent the mean + s.e.m. of two independent experiments. (b, c) Staining of hybridomas expressing the Vα14i TCRα chain paired with wild-type or CDR3β alanine substitutions of the DN32.D3 (b) or 24.8.A (c) TCRβ chains. The MFI of αGC-CD1d tetramer staining was determined for a narrow TCR gate and is normalized to wild-type MFI (set as 100%). Data represents the mean + s.e.m. of two independent experiments.