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. Author manuscript; available in PMC: 2012 Mar 25.
Published in final edited form as: Immunity. 2011 Mar 3;34(3):315–326. doi: 10.1016/j.immuni.2011.01.013

Figure 5.

Figure 5

Grafting of autoreactive CDR3β loop transfers autoreactivity. Staining of hybridomas expressing the Vα14 TCRα chain paired with the indicated Vβ chains in the context of the 2A3-D CDR3β. (a) Representative dot plots of three independent experiments. (b) The MFI of αGC-CD1d tetramer staining was determined for a narrow TCR gate. Data represent the mean + s.e.m. of three independent experiments. (c) Reactivity of the 2A3-D NKT TCR with CD1d molecules. Staining of the 2A3-D hybridoma with tetramers made of mouse CD1d monomers, or mouse CD1d:Ig complexes, produced in the indicated cell lines. Data are representative of three independent experiments.