Figure 3.

CD133 and CD24 expression in renal tissue. A: Quantitative PCR measuring mean relative expression of CD133 mRNA in three paired ALDH^high and ALDH^low cell fractions. Error bars represent SD. n = 3. B–D: The IHC analyses of CD133 protein expression in renal tissue revealed apical CD133 staining (black) in occasional PT cells (arrows) and in cells of the parietal layer of Bowman's capsule (arrowhead in B). Diffuse basolateral positivity was noted in all the tubular cells. The cells were often found in tubular curvatures (C) and sometimes in doublets (D). E and F: Staining with IF demonstrating co-expression of CD133 (red) and CD24 (green). Separate images of the respective fluorochrome channels are shown to the right of the merged images. E: Co-expression of CD24 and CD133 was seen in the parietal cells of Bowman's capsule (arrowhead), which extended down to the urinary pole (star), and in scattered cells of the PTs (arrow). F: A characteristic cell doublet positive for CD24 and CD133 (arrow). G, glomerulus. Scale bars = 200 μm (B and E); 50 μm (C, D, and F).