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. 2011 Feb;178(2):688–698. doi: 10.1016/j.ajpath.2010.10.014

Figure 1.

Figure 1

Generation of the PEDF-tg mice. A: Diagram of the human PEDF transgene. B: Genotyping of Wt (n = 11) and individual PEDF-Tg mice (n = 12). Each lane represents an individual mouse. C: Western blot analysis to confirm overexpression of PEDF. The same amounts of ear protein (50 μg) from F4 to F8 generations of PEDF-Tg and Wt mice were blotted with an anti-PEDF antibody and with β-actin as a loading control. D: High PEDF levels in the serum of PEDF-Tg mice. Total serum proteins (40 μg) from each mouse were analyzed by Western blot analysis using a PEDF-specific antibody and reblotted with an antialbumin antibody. The blot shows results from two representative Wt and PEDF-Tg mice. E: Western blot analysis of PEDF in various tissues from Wt and PEDF-Tg mice. Equal amounts of total proteins from each tissue were blotted with the anti-PEDF antibody.