Table 1.

PAF Accelerates the Skin Disease Phenotype of K5.hTGF-β1 Transgenic Mice

Histologic parameter	Time point and treatment⁎
	24 hours				72 hours
	WT		K5.hTGF-β1		WT		K5.hTGF-β1
	Vehicle	PAF	Vehicle	PAF	Vehicle	PAF	Vehicle	PAF
Skin thickness (μm)	15.1 ± 1.3	18.7 ± 2.0	24.8 ± 2.1	38.7 ± 4.3†	21.7 ± 2.2	26.8 ± 5.5	31.5 ± 3.7	54.4 ± 2.9†
No. of epidermal layers	1.8 ± 0.3	2.0 ± 0.3	3.0 ± 0.2	3.4 ± 0.1	1.6 ± 0.1	2.0 ± 0.2	3.0 ± 0.3	4.0 ± 0.3†
Total dermal cell infiltrate score	0.3 ± 0.1	0.3 ± 0.1	1.5 ± 0.1	2.3 ± 0.2‡	0.3 ± 0.1	0.4 ± 0.2	1.7 ± 0.1	2.5 ± 0.2‡
No. of neutrophils per field (×200)	8.5 ± 1.4	13.8 ± 1.8	55.9 ± 6.0	76.8 ± 9.4	7.8 ± 0.9	18.3 ± 1.9‡	59.8 ± 4.9	93.5 ± 5.0‡

Mice were injected intracutaneously with PAF (100 ng) or with vehicle control. Biopsy samples were collected at injection sites at different time points after injection (24 and 72 hours) for histologic evaluation, as defined in Materials and Methods.

^⁎Data are given as mean ± SEM.

^†P < 0.05.

^‡P < 0.01 (unpaired t-test) comparing the respective PAF versus vehicle treatment values (n = 4 to 8 per treatment and time point).