Figure 2.

Cell proliferation was diminished in XB130 shRNA stably transfected WRO cells. A: Expression of XB130 in shRNA stably transfected WRO cells as determined by Western blotting. shRNA plasmids (C3 and C4) targeting XB130 and a negative control (NC) shRNA plasmid were transfected in WRO cells. Twelve single colonies were established from each transfected cell pool. B: Cell numbers of XB130 shRNA transfected cells (C3-1, C3-4, C4-3, and C4-11) were significantly lower than those of NC shRNA transfected cells (NC-1 and NC-12) at 72 hours after plating. n = 4. Mean ± SEM *P < 0.05 (compared with NC-1). **P < 0.05 (compared with NC-12). C: Cell numbers of C3-1 and C4-3 cells were significantly lower than those of NC-1 cells at 24 hours and 48 hours after plating. n = 4. Mean ± SEM *P < 0.05 (compared with NC-1). D: G₁-S phase progression was significantly diminished in C3-1 and C4-3 cells in comparison with NC-1 cells at 48 hours after plating. Analysis of cell cycle was performed by flow cytometry using PI staining. n = 4. Mean ± SEM *P < 0.05 (compared with NC-1).