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. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: Cell Host Microbe. 2011 Feb 17;9(2):137–146. doi: 10.1016/j.chom.2011.01.010

Figure 1. RIPK interacts with RIN4 and RIPK expression is induced by avrB and avrRpm1.

Figure 1

(A) Maltose Binding Protein (MBP) pulldown between purified recombinant His-RIN4 and MBP-RIPK in vitro. MBP alone is used as a negative control. Proteins were subjected to immunoblot analyses with antibodies recognizing RIN4 and MBP. (B) Quantitative Real Time PCR (qRT-PCR) illustrating RIPK induction upon bacterial inoculation. Four-week-old Arabidopsis leaves were syringe infiltrated with 2.5×107 cfu/ml of Pst DC3000 with the broad host range vector pVSP61carrying empty vector (EV), avrRpm1, or avrB. Y-axis indicates fold change. Error bars represent means ± standard deviation for qRT-PCR, n = 3. See also Figure S1 and Table S1.