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. Author manuscript; available in PMC: 2012 Feb 17.
Published in final edited form as: Cell Host Microbe. 2011 Feb 17;9(2):137–146. doi: 10.1016/j.chom.2011.01.010

Figure 7. RIN4 phosphorylation status alters protein associations.

Figure 7

(A) RIN4 and RIN4 phosphorylation mimic (pRIN4, RIN4(T21E/S160E/T166E)) interaction with AvrB by yeast-two hybrid. Lower panel: immunoblot analyses demonstrating myc-RIN4 and HA-AvrB expression in yeast. (B) AvrB disrupts the RIN4 and RIPK complex in vivo. GFP, T7-RIN4, RIPK-HA, and AvrB-FLAG were transiently expressed in N. benthamiana by Agrobacterium-mediated protein expression. T7-RIN4 was immunoprecipitated with T7 antisera and associated proteins were detected by immunoblot analyses. Bottom panel: Anti-FLAG immunoprecipitation. AvrB-FLAG expression could only be detected by immunoprecipitation in the input due to low-level expression.