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. 2011 Mar 23;121(4):1313–1328. doi: 10.1172/JCI42405

Figure 3. BV resistance is associated with increased EGFR activation on VSCs and the tumor vasculature.

Figure 3

(A) Quantification of EGFR+ cells in H1975 xenografts that progressed on vehicle and BV, using LSC. *P < 0.01, t test. (B) Representative IF staining of CD31 (red), p-EGFR (green), and nuclei (blue) using confocal microscopy in vehicle- and BV-treated H1975 and A549 xenografts at progression. At least 5 microphotographs were collected per sample. Original magnification, ×200. Scale bars: 5 μm (H1975 BV); 10 μm (H1975 vehicle); 20 μm (A549). (C) Percent VSCs and ECs (CD31+) expressing p-EGFR in H1975 and A549 vehicle- and BV-treated tumors at progression. p-EGFR+ cells were counted in at least 5 random microscopic fields for each of 4 samples per group (×200). *P < 0.01, **P < 0.05, t test. (D) Representative IF images of p-EGFR, desmin, and nuclei staining in H1975 vehicle- and BV-treated xenografts at progression. At least 5 microphotographs were collected per specimen. White arrow denotes overlapping p-EGFR, desmin, and nuclei staining in BV-resistant H1975 tumors at higher magnification. Original magnification, ×200; ×400 (magnified merge image). (E) Percent desmin+ cells expressing p-EGFR in vehicle- and BV-treated H1975 tumors at progression. At least 5 random microscopic fields (×200) for each sample were analyzed. *P < 0.01, t test. (A–E) n = 4 per group.