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. 2011 Mar 23;121(4):1313–1328. doi: 10.1172/JCI42405

Figure 8. EGFR is activated in H441 BV-resistant tumors, and dual EGFR/VEGFR inhibition reduces pericyte coverage.

Figure 8

(A) Representative microphotographs of CD31 (red), p-EGFR (green), and nuclei (blue) fluorescent staining in H441 tumors that progressed on vehicle, BV, erlotinib+BV, and vandetanib treatments, using confocal microscopy. At least 5 microphotographs were collected from all the tumor specimens in each group. Original magnification, ×200. Scale bar: 50 μm. (B) Percent p-EGFR fluorescent area in H441 tumors that progressed while on the indicated therapies, as determined using Alpha Innotech Software. 5–10 random microphotographs (×200) of red (CD31), green (p-EGFR), and blue (nuclei) fluorescence were collected from 5 (vehicle and BV), 6 (erlotinib+BV), and 4 (vandetanib) specimens per group. P values were calculated using t test. (C) Representative IF images of CD31, desmin, and nuclei in H441 tumors that progressed while on the indicated treatments, using confocal microscopy. At least 5 microphotographs were collected from all the tumor specimens per group. Original magnification, ×200. Scale bar: 50 μm. (D) Percent pericyte coverage in H441 tumors was quantified in at least 5 microscopic fields (×200) of tumor specimens. n = 4 (erlotinib); 5 (vehicle 2 weeks and vandetanib); 6 (BV 2 weeks and vehicle progression); 7 (BV progression and erlotinib+BV). P values were calculated using t test.