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. Author manuscript; available in PMC: 2011 Oct 1.
Published in final edited form as: Oncogene. 2010 Dec 6;30(16):1947–1955. doi: 10.1038/onc.2010.567

Figure 5.

Figure 5

Release of cytochrome-c from three human hepatoma cells (HepG2, PLC/PRF/5, Hep3B) 12 h after 3 Gy γ-irradiation. In some experiments, HepG2 cells were treated with 20 μm PFT-α before and after irradiation or 1 h with 5 μM CsA before irradiation. (a) Cell image visualized by a fluorescence microscope. Chromatin was stained with 4′,6-diamidino-2-phenylindole and intracellular lipids were stained with Nile red. Immunolocalization of cytochrome-c (green), nuclear (blue) and cell outline morphology (red) were recorded and merged. The size bar in the photo is 20 μm. (b) The percentage of cytochrome-c-released cells in the population of HepG2 cells with and without PFT-α and CsA treatment, respectively. (c) The percentage of cytochrome-c released cells in the population of PLC/PRF/5 and Hep3B cells. *P<0.05 compared with the control without irradiation. ***P<0.001 to the control without irradiation or to the indicated group with inhibitor treatment. Results correspond to the mean±s.e. of three independent experiments with three replicates in each case. A full colour version of this figure is available at the Oncogene journal online.