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. 2011 Apr 4;6(4):e17991. doi: 10.1371/journal.pone.0017991

Figure 2. PCH analysis of TLR9-GFP dimers in live cells.

Figure 2

(a) Brightness values of autofluorescence (Auto), GFP and TLR9-GFP obtained under identical experimental conditions by PCH analysis (n = 12). Blue and red line represent mean brightness counts of autofluorescence and GFP monomer brightness. Black line denotes the predicted brightness of GFP dimers. (b) Percentage of monomer and dimers in TLR9-GFP alone, TLR9 incubated with CpG DNA and TLR9 incubated with non-CpG DNA, quantified by PCH data. Error bars are standard deviation (n = 12).