Figure 3. Cross-correlation analysis of TLR9 with CpG and non CpG DNA.

Confocal fluorescence image of (a) TLR9-GFP (left) with CpG DNA (right) and (c) TLR9-GFP (left) with non CpG DNA (right). Scale bar: 10 µm. Autocorrelation (Blue and red) and cross-correlation (black) curves from FCCS measurement confirms the in vivo binding between TLR9 and CpG (b) or non CpG DNA (d). Curves represent an average of 30 different measurements across 10–30 different cells.