Table 1. Characteristics of Neospora monoclonal antibodies.
mAb | Target site determined by IFA | IFA fixation conditions | MR (kDa) determined by Western blot | Blot conditions |
4C1 | Surface | F | 62 | R |
21H12 | Surface | F | 16 | R |
8H12 | IMC | F | 43 | R |
15D5 | IMC | M | 36 | R |
15G6 | IMC | M | >250 | R |
3D9 | Mitochondrion | F | 33 | R |
4G10 | Mitochondrion | M | 33 | R |
8E10 | Mitochondrion | F | 17, 40 | R |
9G5 | Mitochondrion | F | 33, 60 | R |
14H8 | Mitochondrion | F | UTD | |
10C7 | Apicoplast | F | 60 | R |
10D8 | Apicoplast | M | 60 | R |
2D9 | Rhoptry | F | 36 | R |
6A4 | Rhoptry | F | 80 | R |
11F1 | Rhoptry | F | 60 | R |
11G3 | Rhoptry | F | 34 | R |
12D4 | Rhoptry | M | 65 | R |
16G4 | Rhoptry | F | 36 | R |
17E5 | Rhoptry | F | 36 | R |
18G9 | Rhoptry | F | 34 | R |
20B5 | Rhoptry | F | 65 | R |
20D2 | Rhoptry | F | 65 | R |
10G5 | Rhoptry Neck | M | 68 | R |
10H4 | Rhoptry Neck | M | 22, 62 | NR |
17H12 | Rhoptry Neck | F | 145 | R |
3A5 | Vacuole | F | 15 | R |
4B1 | Vacuole | F | 35 | R |
10B10 | Vacuole | M | 45 | R |
12C1 | Vacuole | F | 36 | R |
16B4 | Vacuole | F | 50 | R |
21H4B | Vacuole | F | 40 | R |
4A4 | Dense Granule | F | 40 | NR |
21H7A | Dense Granule | F | 20 | R |
3D12A | Microneme | F | 72 | R |
10G6 | Microneme | F | 60 | R |
12F5 | Microneme | F | UTD | |
13A2 | Microneme | F | 35 | NR |
13C10 | Microneme | F | UTD | |
15G1 | Microneme | F | 36 | R |
16H9 | Microneme | F | 60 | NR |
18C2 | Microneme | F | UTD | |
21D2 | Microneme | F | 52 | R |
21G11 | Microneme | F | 36 | R |
21H8 | Microneme | F | 35 | NR |
14A4 | Internal spots | F | 32 | R |
17D4B | Internal spots | M | 70 | R |
Abbreviations in the table: F: formaldehyde, M: methanol, NR: non-reduced, R: reduced,
UTD: unable to determine, IMC: inner membrane complex.