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. 2011 Apr 4;6(4):e18383. doi: 10.1371/journal.pone.0018383

Table 1. Characteristics of Neospora monoclonal antibodies.

mAb Target site determined by IFA IFA fixation conditions MR (kDa) determined by Western blot Blot conditions
4C1 Surface F 62 R
21H12 Surface F 16 R
8H12 IMC F 43 R
15D5 IMC M 36 R
15G6 IMC M >250 R
3D9 Mitochondrion F 33 R
4G10 Mitochondrion M 33 R
8E10 Mitochondrion F 17, 40 R
9G5 Mitochondrion F 33, 60 R
14H8 Mitochondrion F UTD
10C7 Apicoplast F 60 R
10D8 Apicoplast M 60 R
2D9 Rhoptry F 36 R
6A4 Rhoptry F 80 R
11F1 Rhoptry F 60 R
11G3 Rhoptry F 34 R
12D4 Rhoptry M 65 R
16G4 Rhoptry F 36 R
17E5 Rhoptry F 36 R
18G9 Rhoptry F 34 R
20B5 Rhoptry F 65 R
20D2 Rhoptry F 65 R
10G5 Rhoptry Neck M 68 R
10H4 Rhoptry Neck M 22, 62 NR
17H12 Rhoptry Neck F 145 R
3A5 Vacuole F 15 R
4B1 Vacuole F 35 R
10B10 Vacuole M 45 R
12C1 Vacuole F 36 R
16B4 Vacuole F 50 R
21H4B Vacuole F 40 R
4A4 Dense Granule F 40 NR
21H7A Dense Granule F 20 R
3D12A Microneme F 72 R
10G6 Microneme F 60 R
12F5 Microneme F UTD
13A2 Microneme F 35 NR
13C10 Microneme F UTD
15G1 Microneme F 36 R
16H9 Microneme F 60 NR
18C2 Microneme F UTD
21D2 Microneme F 52 R
21G11 Microneme F 36 R
21H8 Microneme F 35 NR
14A4 Internal spots F 32 R
17D4B Internal spots M 70 R

Abbreviations in the table: F: formaldehyde, M: methanol, NR: non-reduced, R: reduced,

UTD: unable to determine, IMC: inner membrane complex.