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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Yeast. 2010 Oct 8;28(2):123–136. doi: 10.1002/yea.1824

Figure 2.

Figure 2

CEN/ARS and 2 μ plasmid copy number. Southern blot analysis of cells containing URA3-marked plasmids. DNA was prepared from logarithmic phase cultures containing low- and high-copy URA3-marked vectors and restricted with HindIII. Triplicate samples of 20 μg total DNA for low-copy plasmids and 2 μg total DNA for high-copy plasmids were analysed by Southern blot analysis using a probe specific for PPGK1, as described in Materials and methods. Band intensity was quantitated in the linear range for plasmid and chromosomal fragment hybridization using Quantity One Software (Bio-Rad)