. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: Yeast. 2010 Oct 8;28(2):123–136. doi: 10.1002/yea.1824

Table 2.

List of plasmids created in this study

Plasmid name	Base vector	Description
pXP1	pCR Blunt II	P_PGK₁ with 5³ SspI and 3³ SpeI
pXP2	pCR Blunt II	T_CYC₁ with 5³ SpeI-N₁₂-XhoI and 3³ SspI
pXP3	pCR Blunt II	2 μ with EcoRI flanking sites
pXP4	pCR Blunt II	CEN/ARS ori with EcoRI flanking sites
pXP5	pCR Blunt II	TRP1 marker with loxP repeats and SmaI flanking sites
pXP6	pCR Blunt II	URA3 marker with loxP repeats and SmaI flanking sites
pXP7	pCR Blunt II	CAN1 marker with loxP repeats and SmaI flanking sites
pXP8	pCR Blunt II	MET15marker with loxP repeats and SmaI flanking sites
pXP9	pCR Blunt II	P_PGK₁-T_CYC₁ SspI cassette
pXP13	pUC18	P_PGK₁-T_CYC₁ SspI cassette
pXP16	pCR Blunt II	P_TEF₁ with 53 SspI and 3³ SpeI
pXP17	pUC18	P_TEF₁-T_CYC₁ SspI cassette
pXP100	pXP13	CEN/ARS, P_PGK₁
pXP200	pXP13	2 μ, P_PGK₁
pXP300	pXP17	CEN/ARS, P_TEF₁
pXP400	pXP17	2 μ, P_TEF₁
pXP112	pXP100	P_PGK₁⁻, CAN1⁺, CEN/ARS⁻
pXP212	pXP200	P_PGK₁⁻, CAN1⁺, 2 μ⁻
pXP312	pXP300	P_TEF₁⁺, CAN1⁻, CEN/ARS⁺
pXP412	pXP400	P_TEF₁⁺, CAN1⁺, 2 μ⁻
pXP114	pXP100	P_PGK₁⁻, MET15⁺, CEN/ARS⁻
pXP214	pXP200	P_PGK₁⁻, MET15⁺, 2 μ⁻
pXP314	pXP300	P_TEF₁⁺, MET15⁻, CEN/ARS⁺
pXP414	pXP400	P_TEF₁⁺, MET15⁻, 2 μ⁺
pXP116	pXP100	P_PGK₁⁻, TRP1⁺, CEN/ARS⁻
pXP216	pXP200	P_PGK₁⁻, TRP1⁺, 2 μ⁻
pXP316	pXP300	P_TEF₁⁺, TRP1⁺, CEN/ARS⁺
pXP416	pXP400	P_TEF₁⁺, TRP1⁺, 2 μ⁺
pXP118	pXP100	P_PGK₁⁻, URA3⁻, CEN/ARS⁻
pXP218	pXP200	P_PGK₁⁻, URA3⁻, 2 μ⁻
pXP318	pXP300	P_TEF₁+, URA3⁺, CEN/ARS⁺
pXP418	pXP400	P_TEF₁+, URA3⁺, 2 μ⁺
pXP120	pXP100	P_PGK₁⁻, HIS3⁻, CEN/ARS⁻
pXP220	pXP200	P_PGK₁⁻, HIS3⁺, 2 μ⁻
pXP320	pXP300	P_TEF₁+, HIS3⁺, CEN/ARS⁺
pXP420	pXP400	P_TEF₁⁺, HIS3⁺, 2 μ⁻
pXP122	pXP100	P_PGK₁⁻, LEU2-d8⁻, CEN/ARS⁻
pXP222	pXP200	P_PGK₁⁻, LEU2-d8⁻, 2 μ⁻
pXP322	pXP300	P_TEF₁⁺, LEU2-d8⁺, CEN/ARS⁺
pXP422	pXP400	P_TEF₁⁺, LEU2-d8⁺, 2 μ⁺
pXP518	pBF3055	P_HX_7–391⁺, URA3⁺, CEN/ARS⁻
pXP618	pBF3055	P_HX_7–391⁺, URA3⁺, 2 μ⁺
pXP522	pBF3055	P_HX_7–391⁺, LEU2-d8⁺, CEN/ARS⁻
pXP622	pBF3055	P_HX_7–391⁺, LEU2-d8⁺, 2 μ⁻
pXP114-Rluc	pXP114	6A-Rluc was inserted into SpeI and XhoI sites
pXP214-Rluc	pXP214	6A-Rluc was inserted into SpeI and XhoI sites
pXP116-Rluc	pXP116	6A-Rluc was inserted into SpeI and XhoI sites
pXP216-Rluc	pXP216	6A-Rluc was inserted into SpeI and XhoI sites
pXP118-Rluc	pXP118	6A-Rluc was inserted into SpeI and XhoI sites
pXP218-Rluc	pXP218	6A-Rluc was inserted into SpeI and XhoI sites
pXP318-Rluc	pXP318	6A-Rluc was inserted into SpeI and XhoI sites
pXP418-Rluc	pXP418	6A-Rluc was inserted into SpeI and XhoI sites
pXP518-Rluc	pXP518	6A-Rluc was inserted into SpeI and XhoI sites
pXP618-Rluc	pXP618	6A-Rluc was inserted into SpeI and XhoI sites
pXP120-Rluc	pXP120	6A-Rluc was inserted into SpeI and XhoI sites
pXP220-Rluc	pXP220	6A-Rluc was inserted into SpeI and XhoI sites
pXP122-Rluc	pXP122	6A-Rluc was inserted into SpeI and XhoI sites
pXP222-Rluc	pXP222	6A-Rluc was inserted into SpeI and XhoI sites
pBF3037	pCRBluntII	URA3 was cloned into NheI and MluI flanking by two codon-optimized 200 bp GAG fragments: one was cloned into AscI and NheI sites and the other was cloned into MluI and SalI sites
pKN2735	pYES2	LEU2 replaced URA3
pKN2736	pKN2735	The MCS was modified by introducing NdeI and XmaI
pNB3045	pXP100	LEU2-d8 was flanked by SmaI-loxP on both sides
pBF3038	pKN2736	creA was cloned between NcoI and XmaI
pBF3052	pXP100	P_PGK₁-T_CYC₁ was deleted by SspI digestion
pBF3055	pBF3052	P_HXT_7–391-T_CYC₁was inserted into NdeI and HindIII sites
pBF3060	pBF3038	URA3 replaced LEU2
pBF3187	pXP522	P_TK-Fluc was inserted into NdeI and XhoI sites

Sequence orientation in the pUC18 vector, proceeding 5′ to 3′ clockwise around the map shown in Figure 1.

⁺

Sequence described in Supporting information S3.

⁻

Sequence described in Supporting information S3, orientated with 5′ end distal to the SspI site.