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. Author manuscript; available in PMC: 2011 Apr 4.
Published in final edited form as: Biomaterials. 2009 Dec 5;31(7):1757–1764. doi: 10.1016/j.biomaterials.2009.11.020

Fig. 4. Entry of P85 in primary rat cortical neurons visualized by confocal microscopy.

Fig. 4

(A) Cells exposed to TRITC-P85 (red, 0.001% w/v) for 1 hr and then stained by DRAQ-5 (20 nM, 30 min.) to visualize nucleus. (B) Cells exposed to TRITC-P85 as in panel (A) and then fixed and stained by anti-MAP-2 antibody (10 μg/ml, 4° C overnight) and Alexa 488- labeled secondary antibody. (C) Cells exposed to TRITC-P85 (0.001% w/v) in the presence of Alexa 488-labeled CTB (5 μg/ml) for 60 min, washed and subjected to time lapse live cell imaging after 5 and 30 min.