Fig. 2.
Representative chromatographic profiles of HRP-POx conjugates purification using TSKgel G3000SWXL column (0.78cm × 30 cm): (a) HRP; (b) P(MeOx-b-BuOx); (c) reaction mixture of HRP-P(MeOx-b-BuOx) before purification; (d) HRP-P( MeOx-b-BuOx), after purification. UV absorbance was detected at 220 nm. The mobile phase was methanol (5%) and pH 6.8, 0.1 M NaH2PO4, 0.2M NaCl buffer (95%).