HO1, HO2, VEGF and eNOS protein expression levels in mice brain cortices. Mice were post-treated at 1.5 h of pMCAO with EGb 761 and its constituents, and Western blots were performed to measure the protein expression of HO1, HO2, VEGF and eNOS. The expression of actin was used as a loading control. (A) HO1 expression levels were observed to be significantly increased by the post-treatment of EGb 761 and BB. The expression levels of HO1 were also increased in naïve EGb 761 treated group (non surgery). (B) HO2, known to be constitutively expressed in the brain, was unaffected. (C) VEGF expression levels were significantly increased in EGB 761 and showed an increasing trend in BB treated group. (D) Similarly, eNOS protein expression levels showed significant increase in EGb 761 and BB treated groups. The histograms show the ratio of density captured from HO1, HO2, VEGF, eNOS to that of actin. Values shown are means±SEM from three independent sets of experiments. *p<0.05 vs. corresponding control.