Figure 1.
BRD4-NUT expression is associated with blocked differentiation, and repression of histone acetylation. (A) Effects on histone acetylation resulting from induced expression of flag-BRD4-NUT in 293TRex cells, and siRNA-induced knockdown of BRD4-NUT in two NMC cell lines after 24 hr.. (B) siRNA-induced knockdown of BRD4-NUT using an siRNA targeting NUT in two NMC cell lines. Hematoxylin and eosin staining (H & E) as well as the immunohistochemical (IHC) detection of keratin with antibody AE1/AE3 are shown for each cell line 24h, 48h, and 72h following knockdown. Magnification (scale bar, 25um) is identical for all panels.